OBJECTIVE： To prepare the Indocyanine green gold nanocages （ICG/Biotin-PEG-AuNC-PCM）， and study its antitumor activity. METHODS： Polyol reduction method was used to prepare silver nanometer cubes （AgNC） as template， ion exchange reaction was used to prepare gold nanocages （AuNC） to characterize its morphology， particle size， near infrared （NIR） light and heat properties. Organic solvent evaporation method was conducted to prepare the ICG/Biotin-PEG-AuNC-PCM loaded with ICG， 1-tetradecanol （PCM）， surface modification of biotin polyethylene glycol mercapto （Biotin-PEG-SH）. Its particle size， polydispersity index （PDI） and drug loading were detected， and in vitro cumulative release （Q） within 180 min under 37， 40 ℃ was investigated. Using adriamycin-resistant human breast cancer cells （MCF-7/ADR cells） as objects， MTT method was used to investigate the antitumor activities of ICG， ICG/PEG-AuNC-PCM and ICG/Biotin-PEG-AuNC-PCM， and half inhibitory concentration （IC50） was calculated. RESULTS： AuNC was cubic with particle size of about 60 nm and good light and heat properties. The particle size of ICG/Biotin-PEG-AuNC-PCM was （105±2.8） nm， PDI was 0.261±0.02 （n＝3）. Drug loading was 1.34×108 g/mol AuNC， Q180 min was about 10％ under 37 ℃ and Q20 min was about 80％ under 40 ℃. IC50 of ICG， ICG/PEG-AuNC-PCM and ICG/Biotin-PEG-AuNC-PCM on MCF-7/ADR cells was 95.2， 29.3， 16.1 μg/mL， respectively. CONCLUSIONS： ICG/Biotin-PEG- AuNC-PCM is successfully prepared， and the antitumor activity on MCF-7/ADR cells is stronger than ICG.