OBJECTIVE： To establish a method for simultaneous determination of P-coumaric acid and ferulic acid in the couple of Sparganium stoloni and Curcuma aeruginosa， and to investigate rational proportion of the couple. METHODS： HPLC method was adopted. The determination was performed on Agilent 20RBAX XDB-C18 column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 266 nm， and the column temperature was 30 ℃. The sample size was 10 μL. RESULTS： The linear ranges of P-coumaric acid and ferulic acid were 4.218 6-21.093 μg/ mL（r＝0.999 8）， 1.836 0-9.180 μg/ mL（r＝0.999 9）， respectively. RSDs of precision， stability and reproducibility tests were all lower than 2.0％. The recoveries were 98.72％-100.30％（RSD＝0.18％，n＝9）， 99.11％-100.45％ （RSD＝0.46％，n＝9）. Among 4 compatibility proportions of the couple of S. stoloni and C. aeruginosa （1 ∶ 1，2 ∶ 1，1 ∶ 2，1 ∶ 0，m/m）， the dissolution rate of P-coumaric acid and ferulic acid were the highest when the ratio of S. stoloni-C. aeruginosa （m/m）was 2 ∶ 1. CONCLUSIONS： The method is simple， accurate， stable and reproducible， and can be used for content determination of P-coumaric acid and ferulic acid in the couple of S. stoloni and C. aeruginosa. when the proportion of the couple of S. stoloni and C. aeruginosa is 2 ∶ 1 and the contents of P-coumaric and ferulic acid are the highest，the contents of main etfective components are the highest.