OBJECTIVE： To evaluate the effects of 3 kinds of serum containing blood-activating and stasis-eliminating TCM compound formulas on aggressive behavior， Janus kinase （JAK）/signal transduction and transcriptional activator （STAT） pathway of glioma U251 cells. METHODS： Rats were randomly divided into normal saline group （5 mL/kg）， Taohong Siwu decoction group （5.7 g/kg）， Xuefu Zhuyu decoction （8.5 g/kg） and Didang decoction （2.8 g/kg）， calculated by crude drug， intragastrically administrated once a day， for 10 d. 10％ drug-containing serum culture medium was prepared after 2 h of last administration. After 10％ drug-containing serum culture medium intervening U251 cells for 1 week， Transwell method was conducted to detect the cell invasion rate， Western blot was adopted to detect the metal matrix protease 2 （MMP-2）， MMP-9， phosphorylated JAK2 （p-JAK2）， phosphorylated STAT3 （p-STAT3） protein expression； and real-time fluorescence quantitative polymerase chain reaction method was used to detect MMP-2， MMP-9 mRNA expression. RESULTS： Compared with blank serum， Xuefu Zhuyu decoction drug-containing serum can reduce cell invasion rate （P＜0.05）， decrease the MMP-2， MMP-9 mRNA and its protein expression， and p-JAK2， p-STAT3 protein expression in cells （P＜0.05 or P＜0.01）， while there was no significant difference in above-mentioned indexes in Taohong Siwu decoction drug-containing serum group and Didang drug-containing serum group （P＞0.05）. CONCLUSIONS： In the 3 kinds of blood-activating and stasis-eliminating TCM compound formulas， Xuefu Zhuyu decoction shows significant invasive effect on inhibiting U251 cells； the mechanism may be related to inhibiting the activation of JAK2/STAT3 signal pathway and decreasing MMP-2， MMP-9 gene and protein expressions.