OBJECTIVE： To establish the method for the detection of the known glucagon-like peptide 1 receptor （GLP1R） gene mutation site rs3765467 （NT_007592.16， position： 39 065 819）， and to evaluate its accuracy and practicability. METHODS： Peripheral venous blood samples of 72 healthy subjects were collected in medical examination center of our hospital during Oct. 2015-Feb. 2016. The whole blood DNA was extracted by column extraction method. After amplified by touch down PCR， high resolution melting （HRM） method was adopted to analyze amplified product. Sequencing verification by double stranded chain termination method （Sanger sequencing method） was performed for 38 test samples. The results of 2 methods were compared. RESULTS： The results of mutation scanning showed that there were 39 065 817 and 39 065 819 polymorphism sites in amplified segments. Four types of mutations were detected by HRM method [GCG/GCG， GCA/GCG or ACG/GCG， GCA/GCA or ACG/ACG， A（G）CA（G）]， but 6 types of mutations was detected by Sanger sequencing method [GCG/GCG， ACG/GCG， ACG/ACG， A（G）CA（G）， GCA/GCG， GCA/GCA]. CONCLUSIONS： HRM method can identify GCG/GCG and A（G）CA（G）genotype， but can not identify GCA/GCG and ACG/GCG heterozygous mutation， GCA/GCA and ACG/ACG homozygous mutation. The method is not suitable for the detection of single nucleotide polymorphism for multiple neighboring sites. In the detection of single nucleotide mutation， economical and simple method should be selected after comprehensive analysis of sequence.