OBJECTIVE： To establish the method for simultaneous determination of 5 saponins in Lonicerae Flos. METHODS： Using macranthoidin B as a reference， HPLC method was adopted to calculate the relative correction factor （RCF） of it to macranthoidin A， dipsacoside B， macranthoside A and macranthoside B. The contents of above 4 saponins were calculated through RCF. Using the contents of saponins determined by external standard method as measured value， the calculated value was compared with measured value. RESULTS： The linear ranges of macranthoidin A， macranthoidin B， dipsacoside B， macranthoside A and macranthoside B were 0.316-6.32 μg（r＝0.997 3）， 0.453-9.06 μg（r＝0.998 2），0.231-4.62 μg（r＝0.999 6）， 0.342-6.84 μg（r＝0.998 4）and 0.147-2.94 μg（r＝0.996 1）， respectively. RSDs of precision， stability and reproducibility tests were all lower than 2.0％. The recoveries were 97.74％-104.51％（RSD＝2.37％，n＝6）、96.70％-103.20％（RSD＝2.37％，n＝6）、96.12％-103.61％（RSD＝2.45％，n＝6）、98.80％-104.70％（RSD＝2.32％，n＝6）、99.21％-102.92％（RSD＝1.39％，n＝6）， respectively. There was no statistical significance between calculated value and measured value （P＞0.05）. CONCLUSIONS： The method is simple， precise， stable and reproducible. It can be used for the determination of saponins in Lonicerae Flos.