OBJECTIVE： To find the active ingredient of on antithrombotic chuanxiong rhizoma using computer aided drug design. METHODS： Using “thrombosis” as keyword， thrombosis related proteins were searched and screened in therapeutic target database； target proteins’ three-dimensional structure were downloaded in protein database， then the protein preparing tool were used to determine the coordinates of the active area center. PyRx software and Discovery Studio Visualizer were used to match the 247 small molecules of chuanxiong rhizoma with target protein that downloaded from Taiwan traditional Chinese medicine database. The active molecules were screened and binding force was analyzed. RESULTS： Active molecules of neochlorogenic acid， 1-H-benzimidazole-2-amine， 3， 8-dihydrodiligustilide， chuanxiongterpene were selected by blinding energy， and there were high binding activity among these active molecules， thrombin， antithrombin Ⅲ， coagulation factor Ⅹa and thrombomodulin， and the binding energy were －6.1， －4.5， －7.7， －8.6 kJ/mol. Analysis results showed van Edward force and electrostatic interactions played an important role in their respective docking. CONCLUSIONS： Neochlorogenic acid， 1-H-benzimidazole-2-amine， 3， 8-dihydrodiligustilide， chuanxiongterpene may be the antithrombotic activity ingredients of Chuanxiong rhizoma.