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ZHANG Yan; DONG Lin; YONG Jingjiao; MAO Fuying; YIN Lei; FU Xueyan

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更新时间：2023-03-01

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- China Pharmacy Vol. 28, Issue 21, (2017)
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ZHANG Yan, DONG Lin, YONG Jingjiao, et al. [J]. China Pharmacy 28(21).(2017)
DOI：

ZHANG Yan, DONG Lin, YONG Jingjiao, et al. [J]. China Pharmacy 28(21).(2017) DOI：

摘要

目的：建立同时测定黄芪药材中10种黄酮类成分含量的方法。方法：采用高效液相色谱法。色谱柱为Agilent SB-C18，流动相为乙腈-0.3％甲酸溶液（梯度洗脱），流速为1.0 mL/min，检测波长为254 nm，柱温为35 ℃，进样量为10 μL。结果：毛蕊异黄酮-7-O-葡萄糖苷、异槲皮苷、染料木苷、刺芒柄花苷、毛蕊异黄酮、槲皮素、染料木素、山柰酚、异鼠李素和芒炳花素检测进样量线性范围分别为0.030 29～1.514 5 μg（r＝0.999 4）、0.015 00～0.7 500 μg（r＝0.999 5）、0.007 39～0.369 5 μg（r＝0.999 1）、0.120 11～6.005 5 μg（r＝0.999 8）、0.038 36～1.918 μg（r＝0.999 9）、0.029 89～1.494 5 μg（r＝0.999 5）、0.007 04～0.352 μg（r＝0.999 4）、0.016 83～0.841 5 μg（r＝0.999 5）、0.004 54～0.227 μg（r＝0.999 9）、0.013 36～0.668 μg（r＝0.999 9）；精密度、稳定性、重复性试验的RSD＜2.0％；加样回收率分别为99.55％～100.45％（RSD＝0.36％，n＝6）、99.34％～101.00％（RSD＝0.59％，n＝6）、98.05％～100.36％（RSD＝1.27％，n＝6）、99.73％～100.13％（RSD＝0.18％，n＝6）、99.70％～100.30％（RSD＝0.22％，n＝6）、99.67％～103.27％（RSD＝1.37％，n＝6）、98.13％～104.41％（RSD＝2.37％，n＝6）、96.35％～100.06％（RSD＝1.46％，n＝6）、99.47％～101.13％（RSD＝0.60％，n＝6）、99.70％～100.06％（RSD＝0.15％，n＝6）。结论：该方法操作简便，精密度、稳定性、重复性好，可用于黄芪药材中10种黄酮类成分含量的同时测定。

Abstract

OBJECTIVE： To establish a method for simultaneous determination of 10 flavonoids in Astragalus membranaceus. METHODS： HPLC method was adopted. The determination was performed on Agilent SB-C18 column with mobile phase consisted of acetonitrile-0.3％ formic acid （gradient elution） at the flow rate of 1.0 mL/min. The detection wavelength was set at 254 nm， and the column temperature was 35 ℃. The sample size was 10 μL. RESULTS： The linear ranges of calycosin-7-O-glucoside， isoquercitrin， genistin， ononin， calycosin， quercetin， genistein， kaempferol， isorhamnetin and formononetion were 0.030 29-1.514 5 μg（r＝0.999 4），0.015 00-0.7 500 μg（r＝0.999 5），0.007 39-0.369 5 μg（r＝0.999 1），0.120 11-6.005 5 μg（r＝0.999 8），0.038 36-1.918 μg（r＝0.999 9），0.029 89-1.494 5 μg（r＝0.999 5），0.007 04-0.352 μg（r＝0.999 4），0.016 83-0.841 5 μg（r＝0.999 5），0.004 54-0.227 μg（r＝0.999 9）， 0.013 36-0.668 μg（r＝0.999 9）， respectively. RSDs of precision， stability and reproducibility tests were all lower than 2.0％. The recoveries were 99.55％-100.45％（RSD＝0.36％，n＝6），99.34％-101.00％（RSD＝0.59％，n＝6）， 98.05％-100.36％（RSD＝1.27％，n＝6）， 99.73％-100.13％（RSD＝0.18％，n＝6），99.70％-100.30％（RSD＝0.22％，n＝6），99.67％-103.27％（RSD＝1.37％，n＝6）， 98.13％-104.41％（RSD＝2.37％，n＝6），96.35％-100.06％（RSD＝1.46％，n＝6）， 99.47％-101.13％（RSD＝0.60％，n＝6）， 99.70％-100.06％（RSD＝0.15％，n＝6）， respectively. CONCLUSIONS： This method is convenient， sensitive， stable and reproducible， can be used for simultaneous determination of 10 flavonoids in A. membranaceus.

关键词

黄芪黄酮类成分含量测定高效液相色谱法

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Astragalus membranaceusFlavonoidsContent determinationHPLC

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