OBJECTIVE： To evaluate the effects of clinical pharmacists’ intervention on prophylactic use of antibiotics in removal of internal fixation device for fracture. METHODS： Totally 117 patients underwent removal of internal fixation device for fracture were selected from some on third grade class A hospital during Mar.-Jul. 2015 as pre-intervention group. Totally of 266 patients underwent removal of internal fixation device for fracture were selected during Aug. 2015-May 2016 as post-intervention group. Clinical pharmacists performed interventions based on Rationality Evaluation Criteria for Prophylactic Use of Antibiotics in Removal of Internal Fixation Device for Fracture. The prophylactic use of antibiotics was compared between 2 groups before and after intervention. RESULTS： No unreasonable single dose or additional drug during surgery was found before and after intervention. After intervention， utilization rate of antibiotics for prophylactic use， the proportion of patients receiving antibiotics without indications， the proportion of medication duration more than 24 h decreased from 84.6％， 71.8％， 48.7％ to 17.3％， 9.8％， 4.9％， respectively. Average antibiotics cost， the proportion of total drug cost in hospitalization cost， the proportion of antibiotics cost in total drug cost were all decreased significantly compared to before intervention， with statistical significance （P＜0.05）. There was no statistical significance in the proportion of patients with unreasonable drug choice， unreasonable initial prophylactic medication timing and unreasonable drug combination， the proportion of different antibiotics types， average hospitalization cost and average drug cost （P＞0.05）. CONCLUSIONS： Clinical pharmacists’ interventions significantly reduce the utilization rate of antibiotics for prophylactic use in patients underwent removal of internal fixation device for fracture， improve drug use without indications and rationality of prophylactic use of antibiotics. There still are problems， such as unreasonable medication timing， continuous use time of more than 24 h， etc.  It is necessary to continuous follow-up intervention.