OBJECTIVE： To establish a method for simultaneous determination of pulchinenoside B4， caffeic acid， baicalin， palmatine hydrochloride and berberine hydrochloride in Baipuhuang tablets. METHODS： HPLC method was adopted. The determination of Agela Technologies Venusil XBP C18（L） with mobile phase consisted of acetonitrile-0.05 mol/L monopotassium phosphate （gradient elution） at the flow rate of 0.8 mL/min. The detection wavelengths were set at 203 nm （pulchinenoside B4） and 323 nm （caffeic acid， baicalin， palmatine hydrochloride， berberine hydrochloride）. The column temperature was set at 30 ℃， and the sample size was 10 μL. RESULTS： The linear ranges were 0.081 41-8.141μg for pulchinenoside B4（r＝0.999 8）， 0.018 71-1.871 μg for caffeic acid （r＝0.999 4）， 0.037 33-3.733 μg for baicalin （r＝0.999 2）， 0.028 85-2.885 μg for palmatine hydrochloride （r＝0.999 6） and 0.027 58-2.758 μg for berberine hydrochloride （r＝0.999 7）. The limits of quantitation were 0.009， 0.006， 0.008， 0.011， 0.013 ng， respectively. The limits of detection were 0.030， 0.020， 0.025， 0.034， 0.036 ng， respectively. RSDs of precision， stability and reproducibility tests were all lower than 2.0％. The average recoveries were 97.39％-102.34％（RSD＝1.81％，n＝6）， 96.77％-98.92％（RSD＝0.85％，n＝6）， 97.38％-103.72％（RSD＝2.46％，n＝6）， 96.73％-102.01％（RSD＝2.22％，n＝6）and 96.47％-101.07％（RSD＝1.61％，n＝6）， respectively. CONCLUSIONS： The method is simple， accurate and reproducibility， and can be used for simultaneous determination of pulchinenoside B4， caffeic acid， baicalin， palmatine hydrochloride and berberine hydrochloride in Baipuhuang tablet.