OBJECTIVE： To provide reference for promoting the application of “whole course tutorial system” in the practice teaching of medical higher vocational colleges. METHODS： Totally 106 students on internships of medical higher vocational colleges were selected and randomly divided into control group （n＝53） and observation group （n＝53）. Control group received routine teaching method； observation group received “whole course tutorial system” teaching method on the basis of control group. Score， teaching quality and students’ satisfactory degree were compared between 2 groups after the end of internship teaching. RESULTS：The scores of autonomous learning ability， comprehensive ability， communication ability， judgment ability， thinking and solving problem ability and total score in observation group were all significantly higher than control group after the end of internship teaching； the proportion of good teaching quality in observation group was significantly higher than control group， manifesting as high practical ability， good adaptability， caring patient， good learning condition； the satisfactory degree of students in observation group was significantly higher than control group（98.11％ vs. 88.68％），there was statistical significance（P＜0.05）. CONCLUSIONS： The implementation of “whole course tutorial system” teaching method in practice teaching of medical higher vocational colleges based on conventional teaching method achieves ideal effect and can improve the students’ learning effect， teaching quality and students’ satisfactory degree.