OBJECTIVE： To improve the quality standard of Jianpi zhixiening granules. METHODS： TLC was applied for qualitative identification of Scutellaria baicalensis， Crategi Fructus， Lonicerae japonicae， Codonopsis Radix， Nelumbo nucifera， Coptidis Rhizoma. The contents of berberine hydrochloride and baicalin were determined by HPLC. The determination was performed on Shimadzu VP-ODS column with mobile phase consisted of methanol-acetonitrile-water （46 ∶ 30 ∶ 42， V/V/V， berberine hydrochloride）） （0.1％ sodium dodecylsulphate， 0.1％ phosphoric acid， methanol-0.4％ phosphoric acid （50 ∶ 50，V/V， baicalin） at the flow rate of 1.0 mL/min. The detection wavelengths were set at 265 nm （berberine hydrochloride） and 280 nm （baicalin）. The column temperature was 30 ℃， and sample size was 10 μL. RESULTS： TLC spots of S. baicalensis， Crategi Fructus， L. Japonicae， Codonopsis Radix， N. nucifera， Coptidis Rhizoma were clear and well-separated without negative interference. The linear ranges of berberine hydrochloride and baicalin were 6.67-33.34 μg/mL（r＝0.999 8）and 7.7-38.7 μg/mL （r＝0.999 9）. RSDs of precision， stability and reproducibility tests were all lower than 1.0％. The recoveries were 96.5％-99.9％（RSD＝1.2％，n＝6）and 101.1％-102.9％（RSD＝0.6％，n＝6）. CONCLUSIONS： Improved standard can be used for quality control of Jianpi zhixiening granules.