OBJECTIVE： To establish a method for contents determination of luteoloside， quercetin and hyperoside in Lonicera japonica. METHODS： HPCE was performed silica capillary column with detection wavelength of 360 nm and separation voltage of 20 kV， electrokinetic sampling， sampling voltage of 15 kV， sampling time of 5 s， operation temperature of 25 ℃.The buffer was consisted of 60 mmol/L sodium tetraborate-50 mmol/L natrium carbonicum-50 mmol/L hydroxypropyl-β-cyclodextrin （pH 9.2）. RESULTS： The linear ranges of luteoloside， quercetin and hyperoside were 0.06-0.56mg/mL （r＝0.988 1）， 0.08-0.56 mg/mL （r＝0.989 2）， 0.06-0.49 mg/mL （r＝0.979 6）， respectively. RSDs of precision， stability and reproducibility tests were all lower than 2.0％. The recoveries were 96.12％-99.77％（RSD＝1.29％，n＝6）， 95.90％-98.35％（RSD＝0.89％，n＝6）， 94.07％-97.45％（RSD＝1.33％，n＝6）， respectively. CONCLUSIONS： The method is simple， accurate， stable and reproducible， and can be used for simultaneous determination of luteoloside， quercetin and hyperoside in L. japonica.