OBJECTIVE： To adopt gel method for the determination of bacterial endotoxin in Fat emulsion （10％）/amino acid （15）/glucose （20％） injection. METHODS： According to the gel method in term of “bacterial endotoxin test method” in Chinese Pharmacopeia（2015 edition）， the maximal valid dilution （MVD） of samples were determined through interference test and the validated. The results were compared with chromogenic method. RESULTS： In gel method， the interference to agglutination reaction of TAL and bacterial endotoxin can be excluded when samples were diluted 24 times or less. In chromogenic method， the samples should be diluted 76 times or less. CONCLUSIONS： Gel method can be used for bacterial endotoxin test of Fat emulsion （10％）/amino acid （15）/glucose （20％） injection.