OBJECTIVE： To provide reference for the development and improvement of pharmaceutical care in our hospital. METHODS： By questionnaire investigation， habits and understarding situation and demand of knowledge of drug use among patients in our hospital were randomly collected by face-to-face interview or network platform. A statistical analysis was carried out on obtained results. RESULTS： In this questionnaire investigation， 289 questionnaires were sent out face-to-face， and 282 valid questionnaires were returned with effective feedback rate of 97.58％. Totally 51 valid questionnaires were returned through network platform. A total of 333 valid questionnaires were returned through two ways. The results of questionnaire investigation showed that 44.14％ of the surveyed patients would read the drug instructions before taking drugs； 41.14％ of the surveyed patients would pay more attention to ADR and cautions stated in drug instructions. More than 60％ of the surveyed patients had various bad habits of drug use. When getting better， 66.07％ of the surveyed patients would stop using drugs or reduce the dosage. When having not improved， 26.73％ of them would change drugs or increase dosage. “Consulting with the medical staff ” was the most common source （69.07％） of drug use knowledge， and it was also the most trusted source （84.08％）. Among the knowledge of drug use， surveyed patients most expected to understand the “ADR and side effects” （65.46％）， and the selection “indication and main user”， “cautions”， “drug interaction in multiple use” and “usage and dosage” were also selected frequently， and the choices of some options were significantly affected by the characteristics of people， such as age and education degree （P＜0.05）. The most expected way to acquire drug use knowledge was “face-to-face consultation with medical staff ”（72.97％）， followed by “telephone consultation”and “network consultation”， and the choices of some options were significantly affected by the characteristics of people， such as age and education （P＜0.05）. CONCLUSIONS： Patients in our hospital haven’t pay enough attention to drug instructions and their content， and bad habits of drug use still exist. Sources of drug use knowledge are diverse， but confidence of other sources is not enough except for source of medical staff. The demands for the knowledge of drug use are different， however， the present form and content of pharmaceutical care in our hospital cannot fully meet the needs of patients.