OBJECTIVE： To establish the quality standard for the leaves of Nauclea officinalis. METHODS： Microscopic identification and TLC methods were used for qualitative identification of N. officinalis. The moisture and ash of medicinal materials were determined. HPLC method was adopted to determine the content of strictosamide in medicinal materials. The determination was performed on Lichrospher C18 column with mobile phase consisted of acetonitrile-0.1％ phosphoric acid solution （gradient elution） at the flow rate of 1 mL/min， injection volume was 10 μL. The detection wavelength was set at 226 nm， and the column temperature was 30 ℃. RESULTS： Microscopic identification of the leaves of N. officinalis had strong characteristics， and TLC sports were clear and well-separated without interference from negative control. The linear range of strictosamide were 0.010 5-0.21 mg/mL（r＝0.999 5）； RSDs of precision， stability and reproducibility tests were all lower than 2.0％. Average recoveries were 96.25-101.82％（RSD＝1.86％，n＝9）. Total ash of medicinal materials was 5.27％-6.44％， acid insoluble ash was 0.23％-0.36％，water content was 9.48％-11.46％， strictosamide was 0.124％-1.003％. CONCLUSIONS： Established standard can be used for quality evaluation of N. officinalis.