OBJECTIVE： To explore the effects of extracts from Siegesbeckiae herba （HS） on doxorubicin （DOX）-induced chronic myocardial injury in mice. METHODS： 48 mice were randomly divided into 3 groups. Mice in blank control （Con） group received distilled water once every day， ig， and normal saline （2 mL/100 g） once every day， ip， for 8 weeks； mice in DOX mice received distilled water （2 mL/100 g） once every day， ig， and DOX （3 mg/kg） once every week， ip， for 8 weeks； mice in DOX+HS group received HS （340 mg/kg） once every day， ig， and DOX （3 mg/kg） once every week， ip， for 8 weeks. After administration， body mass， heart coefficient， cardiac function changes， serum biochemical index levels [alanine aminotransferase （ALT）， aspartate aminotransferase （AST）， creatine phosphokinase （CK）， creatine kinase isoenzyme （CK-MB）， lactate dehydrogenase （LDH） and total cholesterol （TC）] of mice were determined. RESULTS： Compared with Con group， body mass of mice in DOX group was decreased （P＜0.01）； heart coefficient was increased （P＜0.05）； heart rate slowed down， R-wave was increased （P＜0.05 or P＜0.01）； serum biochemical indexed were increased， there was significant difference in AST （P＜0.01）. Compared with DOX group， heart coefficient of mice in DOX+HS group was decreased （P＜0.01）； heart rate was increased （P＜0.01）； serum biochemical indexes were decreased， there was significant differences in CK， LDH， TC （P＜0.01）. CONCLUSIONS： HS has certain protective effects on DOX-induced chronic myocardial injury in mice.