OBJECTIVE： To determine the content of phenolic acids from Anemone altica， and optimize its extraction technology. METHODS： HPLC was used to determine the contents of mono-ferulyl tartaric acid and ferulic acid from A. altica； using the total contents of 2 index components as index， volume fraction of extraction solvent， extraction solvent volume， extraction times and extraction time as factors， orthogonal test was used to optimize extraction technology， and verification test was conducted. RESULTS： The contents of mono-ferulyl tartaric acid and ferulic acid were 0.059％， 0.002 5％， respectively； the optimal extraction technology was as follow as 30％ ethanol 600 mL added to 20 g medicinal material， extracted twice， 90 min every time. In verification test， the average contents of 2 components in extract were 0.297 1％ （RSD＝3.64％， n＝3）， 0.004 1％ （RSD＝5.11％， n＝3）. CONCLUSIONS： A method for contents determination of mono-ferulyl tartaric acid and ferulic acid from A. altica is established； optimized extraction technology is stable and feasible.