OBJECTIVE： To provide the basis for the formulation of the health intervention strategy for medical and health institutions’ pharmacists and provide reference for other related research. METHODS： A total of 180 medical and health institutions’ pharmacists in Heilongjiang province were investigated and evaluated by work satisfaction MMSS scale （modified） and quality of life （SF-36） scale， and the relevant data were analyzed statistically. RESULTS： A total of 180 questionnaires were distributed and 134 valid questionnaires were collected. The effective recovery rate was 74.44％. The average score of work satisfaction degree was （3.44±0.79）， which was at the middle level. The highest score was the relationship with colleagues and the lowest score was welfare. The physical function， the physiological function and the social function dimension of the respondents were higher than “Chinese norm”. The scores of physical pain， general health， vitality， emotional function and mental health were lower than “Chinese norm”， and there was statistical significance between the scores of the other dimensions and “Chinese norm” （P＜0.05）， except for the physiological function and vitality dimension. Multivariate analysis showed that gender， duties， education， length of service and work satisfaction score had an effect on quality of life （P＜0.05）， among these factors， work satisfaction score was positively correlated with quality of life. CONCLUSIONS： The quality of life of medical and health institutions’ pharmacists still needs to be improved， which is related to many influential factors， and job satisfaction is an important influential factor.