OBJECTIVE： To observe therapeutic efficacy and safety of Duhuo jisheng decoction combined with glucosamine gulfate for genual osteoarthritis. METHODS： Totally 86 patients with genual osteoarthritis selected from our hospital during Dec. 2015-Apr. 2016 were divided into trial group and control group according to random number table， with 43 cases in each group. Control group was given Glucosamine sulfate tablets 1-2 tablet， po （at mealtime）， tid. Trial group was additionally given Duhuo jisheng decoction， one dose a day， decocting 200 mL with water， morning and evening at twice， on the basis of control group. Both groups received treatment for 28 d. Clinical efficacies of 2 groups were observed as well as the levels of hs-CRP， TNF-α， IL-6， MMP-3， MMP-9 and MMP-13. The occurrence of ADR was recorded during treatment. RESULTS： Before treatment， there was no statistical significance in hs-CRP， TNF-α， IL-6， MMP-3， MMP-9 and MMP-13 between 2 groups （P＞0.05）. After treatment， total response rate of trial group （95.3％） was significantly higher than that of control group （76.7％）， with statistical significance （P＜0.05）. The level of hs-CRP in trial group was significantly lowered， and the levels of TNF-α and IL-6， MMP-3， MMP-9， MMP-13 in 2 groups were decreased significantly； above indexes of trial group were significantly lower than those of control group， with statistcal significance （P＜0.05）. The incidence of ADR in trial group （7.0％） was significantly lower than control group （46.5％）， with statistical significance （P＜0.05）. CONCLUSIONS： Duhuo jisheng decoction combined with glucosamine gulfate shows good therapeutic efficacy for genual osteoarthritis with good safety.