OBJECTIVE： To study the purification technology of Sanhuang yishen formula. METHODS： Using retention rate and impurity rate of purified total polysaccharide， astragaloside and calycosin glucoside as index， the purification effects of water extraction and alcohol precipitation method （50％， 60％， 70％ ethanol） and clarifying agent method （101 juice clarifying agent， ZTC natural clarifying agent， chitosan clarifying agent） were respectively detected to screen the purification method； orthogonal test was used to optimize the technology parameters （mass concentration of liquid， amount of clarifying agent and pH of liquid） by the optimized purification method， and the verification test was conducted. RESULTS： The purification was better when using chitosan as clarifying agent with comprehensive score of 98.62； the purified technology parameters were mass concentration of liquid 1 g/mL， 1％ chitosan solution amount of 2 mL/g， pH 5.1； the average value of retention rate and impurity rate of purified total polysaccharide， astragaloside and calycosin glucoside in verification test were 79.56％ （RSD＝1.24％， n＝3）， 78.11％ （RSD＝0.97％， n＝3）， 79.46％ （RSD＝1.03％， n＝3） and 32.18％ （RSD＝1.16％， n＝3）， respectively. CONCLUSIONS： Using chitosan as clarifying agent shows good purification effect for Sanhuang yishen formula， which is simple. The optimized technology is stable and feasible.