OBJECTIVE： To establish a method for the simultaneous determination of rutin， hesperidin， arctiin， liquiritin and glycyrrhizinate in Sangxing oral liquid. METHODS： RP-HPLC was performed on the column of Kromasil C18 with mobile phase of acetonitrile-0.1％ phosphoric acid solution（gradient elution） at a flow rate of 1.0 mL/min， the detection wavelength was 276， 348 nm， column temperature was 30 ℃，and the injection volume was 5 μL. RESULTS： The linear range was 1.068-10.68 μg/mL（r＝0.999 3） for rutin， 9.320-93.20 μg/mL（r＝0.999 2） for hesperidin， 46.91-469.1 μg/mL（r＝0.999 4） for arctiin， 0.511 0-5.110 μg/mL（r＝0.999 2） for liquiritin； RSDs of precision， stability and reproducibility tests were less than 3％；recoveries were 95.10％-101.90％（RSD＝2.1％，n＝9），96.75％-101.80％（RSD＝1.4％，n＝9），95.69％-100.00％（RSD＝1.5％，n＝9）， 98.22％-104.60％（RSD＝2.1％，n＝9）， respectively. CONCLUSIONS： The method is accurate， sensitive and reproducible， and can be used for the simultaneous determination of rutin， hesperidin， arctiin and liquiritin in Sangxing oral liquid.