OBJECTIVE： To provide reference for promoting rational use of vancomycin in the clinic. METHODS： Referring to vancomycin instruction，Guiding Principles for Clinical Use of Antibacterials （2015 edition） and Chinese Expert Consensus on Clinical Use of Vancomycin （2011 edition）， 720 inpatient medical records of vancomycin were collected from our hospital during Jan. 2013-Dec. 2014， and then drug use of those inpatients were analyzed retrospectively. RESULTS： Among 720 cases， male （428 cases） was more than female （292 cases）， and most of them aged 41-65 （45.83％）. There were 50 cases of prophylactic drug use （6.94％） and 670 cases of therapeutic drug use （93.06％）. The patients came from 36 departments. Among 720 cases， the dose of 19 cases were higher than 2 g/d， and there were 13 ADR cases （1.81％）. Among 670 cases of therapeutic drug use， 587 cases of microbiological samples （87.61％） were detected， and other antibacterials were used in 522 cases additionally （77.91％）. DUI of vancomycin was equal to 0.96 in 549 cases no younger than 18 years old of herapeutic drug use. There were 240 cases of unsuitable drug use （33.33％） in total， including 192 cases of unsuitable solvent （26.67％）， 28 cases of unsuitable usage and dosage （3.89％） and 20 cases of unsuitable drug combination （2.78％）. CONCLUSIONS： The use of vancomycin in our hospital is basically rational；  vancomycin is widely used in departments； drug combination is a common phenomenon； the inspection rate of microbiological samples is qualified； no drug abuse is found. However， there still is inappropriate use in the clinic. It is recommended to strengthen special evaluation and training about rational use of vancomycin， and further standardize monitoring for vancomycin use so as to guarantee the safety of drug use.