OBJECTIVE： To study the chemical constituents in antioxidant activity part of Semiaquilegia adoxoides. METHODS： The antioxidant activity part of S. adoxoides were isolated and purified by chromatography on silicagel and Sephadex LH-20 column， and compound structures were identified physicochemical properties and spectral data analysis. RESULTS： Nine compounds were isolated from the antioxidant activity part of S. adoxoides， namely magnoflorine （1）， griffonilide （2）， salidroside （3）， ferulic acid （4）， genistein （5）， 2，4-dihydroxybenzoic acid （6）， chlorogenic acid （7）， caffeic acid （8） and p-coumaric acid （9）. CONCLUSIONS： The study confirms the active material basis in antioxidant activity part of S. adoxoides for the first time.