OBJECTIVE： To prepare anti-tumor drug H6 （lactone compound） polymeric micelles， and to investigate its in vitro anti-tumor effects. METHODS： Using mPEG2000-PCL4000 as carrier， H6/mPEG2000-PCL4000 micelles were prepared. Using particle size， PDI and 48 h whether to produce precipitation as indexes， feeding ratio， H6 concentration， volume ratio of organic solvent were screened. The encapsulation efficiency and drug-loading amount of micelle were all detected. MTT assay was used to detect the toxicity of micelles and H6 solution to human non-small cell lung cancer cell A549 and human lung cancer cell H460. RESULTS： The screened formulation was as follows as feeding ratio of 1 ∶ 25， H6 concentration of 2 mg/mL， the ratio of ethanol to chloroform of  1 ∶ 1 （V/V）. The parameters of prepared H6/mPEG2000-PCL4000 micelles were as follows as particle size of （40.74±0.116 3） nm， PDI of （0.101±0.006）， encapsulation efficiency of （94.87±0.016 3）％， drug-loading amount of （7.07±0.001 5）％ （n＝3）. IC50 of micelles and H6 solution to A549 cell were 15.62 and 12.57 nmol/L； IC50 of micelles and H6 solution to H460 cell were 27.68 and 15.19 nmol/L. CONCLUSIONS： H6/mPEG2000-PCL4000 micelles are prepared successfully and show in vitro anti-tumor effects.