OBJECTIVE： To establish a method for the contents determination of salidroside and polydatin in new Fuzheng chuyi granule. METHODS： RH-HPLC was performed on the column of GRACE Alltima TMC 18 with mobile phase of methanol - water （15 ∶ 85，V/V，salidroside） and acetonitrile-water （23 ∶ 77，V/V，polydatin） at a flow rate of 1.0 mL/min， detection wavelength was 275 nm （salidroside） and 306 nm （polydatin）， column temperature was 25 ℃， and the injection volume was 10 μL. RESULTS： The linear range was 0.99-10.89 μg for salidroside （r＝0.999 8） and 0.046-1.196 μg for polydatin （r＝0.999 8）； RSDs of precision， stability and reproducibility tests were lower than 2.0％； recoveries were 96.31％-99.44％ （RSD＝1.20％，n＝6） and 95.96％-99.73％ （RSD＝1.42％，n＝6）. CONCLUSIONS： The method is simple， accurate and reliable， and suitable for the contents determination of salidroside and polydatin in new Fuzheng chuyi granule.