OBJECTIVE： To investigate the effects of NOS1AP rs12742393 A/C gene polymorphism on lipid-regulating response of rosuvastatin calcium. METHODS： Two hundred and thirty six patients with coronary heart disease （CHD） were selected from cardiology department of our hospital during Jan. 2014-Jun. 2015， and then given rosuvastatin calcium and other symptomatic treatment for 12 weeks. Polymorphism of NOS1AP rs12742393 A/C was detected by PCR-RFLP. The serum levels of TG， TC， HDL-C and LDL-C were detected by photoelectric colorimetry before treatment and 4， 12 weeks after treatment. The serum serum relationship of genotype with the level of blood lipid was analyzed. RESULTS： Among 236 CHD patients， there were 131 cases of AA genotype（55.5％）， 98 cases of AC genotype（41.5％） and 7 cases of CC genotype（3.0％）； genotype and allele frequencies met the Hardy-Weinberg balance （P＞0.05）. There were 132 patients with normal blood lipid and 104 patients with hypercholesterolemia； there was statistical significance in genotype and allele frequencies （P＜0.05）. Among 104 CHD patients with hypercholesterolemia before treatment， there was no statistical significance in the levels of TG， TC， LDL-C and HDL-C between AA genotype and AC+CC genotype （P＞0.05）. 4th and 12th week after treatment， the levels of TG， TC and LDL-C in different genotypes were all decreased significantly； 4th week after treatment， the level of LDL-C in AC+CC genotype was significantly lower than AA genotype， and the change compared to before treatment was significantly more than AA genotype， with statistical significance （P＜0.05）. There was no statistical significance in the level of HDL-C among different genotypes compared to before treatment； there was no statistical significance in the levels of TG， TC and HDL-C 4th， 12th week after treatment and their changes compared to before treatment between AA genotype and AC+CC genotype； there was no statistical significance in the level of LDL-C 12th week after treatment and their changes between AA genotype and AC+CC genotype（P＞0.05）. CONCLUSIONS： NOS1AP rs12742393 A/C gene polymorphism is associated with CHD complicated with hypercholesterolemia； the C allele of NOS1AP rs12742393 may strengthen the response of CHD patients with hypercholesterolemia to rosuvastatin calcium through influencing the level of LDL-C.