OBJECTIVE： To investigate the protective effect of the compatibilities of ginsenosides Rg1 and aconitine on myocardial cell of in vitro cultured heart failure model. METHODS： The myocardial cells of neonate rat were grouped into normal control group， model group， positive control group （Deslanoside injection， 1×10－7 mol/L）， ginsenosides Rg1 group （1×10－8 mol/L）， aconitine group （1×10－9 mol/L） or their compatibilities groups （1 ∶ 1，2 ∶ 1，1 ∶ 2， V/V）. Except for normal control group， other groups were given 0.8％ pentobarbital sodium to induce heart failure model of myocardial cells. After modeling， each group was given relevant medicine for 1 h， and then the activities of T-ATPase， Ca2+-Mg2+-ATPase， Na+-K+-ATPase in cells were all detected. The activities of acyl carrier protein （ACP） and lactate dehydrogenase （LDH）， and the contents of brain natriuretic party（BNP）， TNF-α and total glycogen were measured in cell culture fluid. RESULTS： Compared with normal control group， T-ATPase and Ca2+-Mg2+-ATPase activities were decreased significantly in model group； meanwhile， Na+-K+-ATPase activity was increased significantly， and ACP， LDH activities and BNP content in cell culture fluid were increased significantly （P＜0.05）. Compared with model group， the activities of T-ATPase in treatment groups were increased significantly， while the activity of LDH in cell culture fluid was decreased significantly； when the volume ratio of ginsenoside Rg1 to aconitine was 2 ∶ 1， protective effect was the strongest； the activities of Na+-K+-ATPase in aconitine group and compatibility groups were all decreased significantly， with statistical significance （P＜0.05）. The activities of ACP and BNP in cell culture fluid were all decreased in treatment groups， but the content of total glycogen in cells and the TNF-α content of in cell culture fluid had no change （P＞0.05）. CONCLUSIONS： Compatibility of ginsenosides Rg1 and aconitine can improve ATPase activities and membranous permeability， regulate BNP secretion and protect myocardial cell of heart failure model， especially the compatibility of ginsenosides Rg1 to aconitine of 2 ∶ 1 ratio.