OBJECTIVE： To establish a method for simultaneous determination of paeoniflorin and salvianolic acid B in Ruangan suopi pill. METHODS： HPLC was performed on the column of Wondasil C18 with mobile phase of acetonitrile -0.1％ phosphoric acid solution （gradient elution） at a flow rate of 1.0 mL/min， the detection wavelength was 230 nm for paeoniflorin and 286 nm for salvianolic acid B， the column temperature was 30 ℃， and the injection volume was 10 μL. RESULTS：The linear range was 9.79-195.80 μg/mL（r＝0.999 9） for paeoniflorin， 11.45-229.00 μg/mL（r＝0.999 9） for salvianolic acid B； the limits of quantification were 0.018 1，0.014 4 μg， limits of detection were 0.005 4， 0.004 3 μg； RSDs of precision， stability and reproducibility tests were lower than 2.0％； the recoveries were 98.67％-102.22％（RSD＝1.26％，n＝9） and 99.72％-103.28％（RSD＝1.05％，n＝9）. CONCLUSIONS： The method is rapid， sensitive and accurate， and can be used for the simultaneous determination of paeoniflorin and salvianolic acid B in Ruangan suopi pill.