OBJECTIVE： To study the anti-hepatic fibrosis effects of Yidu tiaogan mixture on rats and its mechanism. METHODS： 60 SD rats were randomly divided into normal group， model group， colchicine group （positive control， 0.2 mg/kg） and Yidu tiaogan mixture low-dose， medium-dose and high-dose groups （2.7， 5.4， 10.8 mL/kg）. Except for normal group， liver fibrosis model was induced in other groups， and they were given relevant medicine intragastrically during modeling， once a day， for 8 weeks. Serum levels of ALT， AST， HA， PCⅢ， CⅣ， TIMP-1， MMP-1， MMP-2， MMP-13， TGF-β1 and TNF-α and the levels of Hyp， SOD， GSH-Px， CAT， MDA， TGF-β1 and TNF-α in liver tissue were detected in rats. RESULTS： Compared with normal group， the serum levels of ALT， AST， HA， PCⅢ， CⅣ， TIMP-1， MMP-2， TGF-β1 and TNF-α were increased significantly in model group， while the serum levels of MMP-1 and MMP-13 were decreased significantly （P＜0.05）； the levels of Hyp， TGF-β1， TNF-α and MDA in liver tissue were increased significantly， while the levels of SOD， GSH-Px and CAT were decreased significantly （P＜0.05）. Compared with model group， the serum levels of ALT， AST， HA， PCⅢ， CⅣ， TIMP-1 and TNF-α as well as the levels of Hyp， TGF-β1， TNF-α and MDA in liver tissue were all decreased significantly of rats in Yidu tiaogan mixture medium-dose，high-dose and colchicine groups （P＜0.05）； the serum levels of MMP-1 and MMP-13 as well as the level of CAT in liver tissue were increased significantly in Yidu tiaogan mixture high-dose group and colchicine group （P＜0.05）， while the serum levels of MMP-2 and TGF-β1 were decreased significantly （P＜0.05）. There was no statistical significance in above indexes in Yidu tiaogan mixture low-dose group （P＞0.05）. CONCLUSIONS： Yidu tiaogan mixture exerts protective effects on liver fibrosis of rat through regulating TIMP/MMP balance， suppressing oxidative stress， decreasing the level of TGF-β1 and reducing deposition of extracellular matrix in liver.