OBJECTIVE： To establish a method for the simultaneous determination of 7 active constituents in Tangshen qingdu granule. METHODS： HPLC was performed on the column of SHIMADZU Inert Sustain C18 with mobile phase of acetonitrile- 0.1％ phosphoric acid at a flow rate of 1.0 mL/min， detection wavelength was 327 nm for chlorogenic acid and caffeic acid， 280 nm for baicalin， 228 nm for arctiin and 276 nm for wogonoside， baicalein and wogonin， column temperature was 35 ℃， and injection volume was 10 μL. RESULTS： The linear range was 4.830-154.6 μg/mL for chlorogenic acid and （r＝0.999 8）， 0.750-24.1 μg/mL for caffeic acid（r＝0.999 7），22.859-731.5 μg/mL for baicalin（r＝0.999 7），8.491-271.7 μg/mL for arctiin （r＝0.999 3）， 2.471-79.0 μg/mL for wogonoside（r＝0.999 6），6.656-213.0 μg/mL for baicalein（r＝0.999 4） and 2.756-88.2 μg/mL for wogonin （r＝0.999 8）； RSDs of precision， stability and reproducibility tests were lower than 2.0％， recoveries were 96.86％-100.82％ （RSD＝1.46％，n＝6）， 98.79％-101.09％（RSD＝0.93％，n＝6）， 97.57％-101.51％（RSD＝1.37％，n＝6）， 97.76％-99.63％ （RSD＝0.77％，n＝6）， 97.99％-100.12％（RSD＝0.76％，n＝6）， 96.54％-101.07％（RSD＝1.87％，n＝6） and 96.60％-99.59％ （RSD＝1.14％，n＝6）. CONCLUSIONS： The method is simple with good precision， stability and reproducibilty， and can be used for the simultaneous determination of 7 active constituents in Tangshen qingdu granule.