OBJECTIVE： To analyze the association of antituberculosis drug-induced hepatotoxicity （ADIH） with gene polymorphisms of cyptochrome P450（CYP）2E1 and glutathione-S-transferase （GST）M1， and to discuss susceptibility factor. METHODS： 91 southern and Han inpatients with tuberculosis were selected from our hospital during Oct. 2011-Jun. 2015. According to the occurrence of ADIH， those patients were divided into ADIH group （34 cases） and non-ADIH group （57 cases）. Peripheral venous blood of patients were collected， PCR and multiple PCR were used to detect CYP2E1 and GSTM1 gene polymorphisms. The susceptibility factors of ADIH was investigated by Logistic regression analysis. RESULTS： CYP2E1 genotype of all patients were c2/c2 genotype （100％）， and GSTM1 genotype distribution met Hardy-Weinberg balance （P＞0.05）. There were 9 patients （26.5％） with GSTM1（+/－）genotype and 25 patients （73.5％） with GSTM1（－/－） genotype in ADIH group， with statistical significance （P＜0.05）. There were 17 patients （29.8％） with GSTM1（+/－）genotype and 40 patients （70.2％） with GSTM1（－/－） genotype in non-ADIH group， with statistical significance （P＜0.05）. There was no statistical significance in the distribution of GSTM1（+/－）and（－/－）genotype between 2 groups （P＞0.05）. Logistic regression analysis showed that there was no correlation of gender， age， BMI and GSTM1 genotype with ADIH （P＞0.05）. CONCLUSIONS： Gender， age， BMI and GSTM1 genotype may be not correlated with ADIH； The relationship of GSTM1 genotype with ADIH can not be judged because only one kind of CYP2E1 genotype has been detected.