OBJECTIVE： To investigate the hemostasis， analgesic， anti-inflammatory and anti-bacterial effect of Zhuang medicine Hemorrhoids fumigant. METHODS： KM mice （or SD rats） were randomly divided into model control group， Compound schizonepeta lotion group [positive drug， 0.56 g （crude drug）/kg] and Hemorrhoids fumigant low-dose， medium-dose and high- dose groups [2.40， 4.80， 9.60 g （crude drug）/kg] according to body mass， with 10 rats or mice in each group， for external administration. The hemostasis， analgesic and anti-inflammation effects of Hemorrhoids fumigant were investigated through determining tail bleeding time， 0.6％ acetic acid-writhing times and xylene-induced ear swelling of mice and carrageenin-induced paw swelling of rats. The broth two times dilution method was used to determine minimal inhibitory concentration （MIC） and minimum bactericidal concentration （MBC） of Hemorrhoids fumigant to Pseudomonas aeruginosa （PA）， Staphylococcus epidermidis （SE）， Escherichia coli （EC）， Monilia albicans （MA）， Staphylococcus aureus （SA）， Salmonella paratyphi B （SPB）， Bacillus subtilis （BS） and Klebsiella pneumoniae （KP）. RESULTS： Compared with model control group， the tail bleeding time， writhing times and the degree of ear swelling in mice were decreased in treatment groups， and the degree of paw swelling in rats was decreased in treatment groups， with statistical significance （P＜0.05 or P＜0.01）. MIC of Hemorrhoids fumigant to PA， SE， EC， MA， SA， SPB， BS， KP were 0.045， 0.091， 0.091， 0.181， 0.091， 0.091， 0.091， 0.181 g/ml， respectively； corresponding MBC were 0.091， 0.181， 0.181， 0.363， 0.091， 0.181， 0.181， 0.181 g/ml， respectively. CONCLUSIONS： Hemorrhoids fumigant shows obvious hemostasis， analgesic， anti-inflammatory and anti-bacterial effects.