OBJECTIVE： To observe the effects of Sodium chloride injection and Glycerine enema on the relief of postoperative defecation pain， edema around wound and incision in patients with complex anal fistula. METHODS： 100 patients with complex anal fistula were selected and divided into group A （33 cases）， B （33 cases） and C （34 cases） according to random number table. Group A was given Sodium chloride injection 300-500 ml， water bath heated to 35-37 ℃， into the rectum via enema bag before defecation， and defecated after controlling defecation for 5-10 min. Group B was given Glycerine enema 110 ml into the rectum， and defecated after controlling defecation for 5-10 min. Group C could defecated directly when they felt obvious defecation desire. VAS score of initial defecation， defecation frequence and time within 24 h after initial defecation， edema around wound and incision within a week after initial defecation were observed in 3 groups as well as the occurrence of ADR. RESULTS： VAS score of initial defecation， defecation frequence and time， edema around wound and incision in group A and B were significantly lower/shorter than in group C， with statistical significance （P＜0.05）. Above indexes of group B were slightly lower than those of group A， without statistical significance （P＞0.05）. No obvious ADR was found in group A and B during treatment. CONCLUSIONS： Both Sodium chloride injection and Glycerine enema can relieve postoperative defecation pain in patients with complex anal fistula， and reduce edema around wound and incision with good safety. The two drugs should be selected according to patient’s condition.