OBJECTIVE： To establish a method for the contents determination of dipsacoside B， luteolin and apigenin in Zang medicine Lamiophlomis rotata. METHODS： HPLC was performed on the column of Diamonds C18 with mobile phase of acetonitrile-0.1％ phosphoric acid solution （gradient elution） at a flow rate of 1.0 ml/min， detection wavelength was 238 nm for dipsacoside B， 348 nm for luteolin and apigenin， column temperature was 25 ℃， and injection volume was 10 μl. RESULTS： The linear range was 3.08-308 μg/ml for dipsacoside B （r＝0.999 8）， 3.72-372 μg/ml for luteolin （r＝0.999 9）and 2.92-292 μg/ml for apigenin（r＝0.999 9）； RSDs of precision， stability and reproducibility tests were lower than 3.0％； recoveries were 95.38％-103.15％ （RSD＝1.85％，n＝9）， 96.05％-102.66％（RSD＝2.07％，n＝9） and 95.49％-103.59％（RSD＝2.18％，n＝9）. CONCLUSIONS： The method is simple with good precision， stability and reproducibility， and can be used for the simultaneous determination of dipsacoside B， luteolin and apigenin in Zang medicine L. rotata.