OBJECTIVE： To establish a method for the simultaneous determination of chlorogenic acid， hesperidin and baicalin in Shubiaoling granule. METHODS： HPLC was performed on the column of SAGA Tri-Sal C18 with mobile phase of acetonitrile - 0.5％ phosphoric acid （gradient elution） at a flow rate of 1.0 ml/min， detection wavelength was 320 nm， column temperature was 30 ℃， injection volume was 10 μl. RESULTS： The linear range was 2.463-240.5 μg/ml for chlorogenic acid （r＝0.999 9）， 6.577-642.3 μg/ml for hesperidin （r＝0.999 8） and 1.708-166.8 μg/ml for baicalin （r＝0.999 8）； RSDs of precision， stability and reproducibility tests were no more than 1.8％； recoveries were 97.02％-102.40％ （RSD＝1.9％，n＝6），96.55％-100.20％（RSD＝1.6％，n＝6） and 97.51％-100.70％ （RSD＝1.2％，n＝6）， respectively. CONCLUSIONS： The method is simple， accurate with high sensitivity and good reprosucibility， and can be used for the simultaneous determination of chlorogenic acid， hesperidin and baicalin in Shubiaoling granule.