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LYU Xiaohui; WANG Zhe; SUN Jiayu; CAI Zhigang; ZHANG Ruipeng; SHAN Aiyun

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更新时间：2023-03-01

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- China Pharmacy Vol. 27, Issue 31, (2016)
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LYU Xiaohui, WANG Zhe, SUN Jiayu, et al. [J]. China Pharmacy 27(31).(2016)
DOI：

LYU Xiaohui, WANG Zhe, SUN Jiayu, et al. [J]. China Pharmacy 27(31).(2016) DOI：

摘要

目的：探讨六味地黄丸对糖尿病模型大鼠血管中血红素加氧酶1（HO-1）的影响。方法：将大鼠随机分为正常组、模型组、六味地黄丸组（14 g/kg，每天ig给药）和HO-1抑制剂锌原卟啉（10 μmol/kg，隔日ip给药）+六味地黄丸组（14 g/kg，每天ig给药），每组12只。除正常组外，其余各组大鼠均ip链脲佐菌素诱导糖尿病模型。成模后，各给药组大鼠给予相应药物，正常组和模型组大鼠隔日ip生理盐水，连续4周。检测大鼠尾动脉收缩压（SBP）和心率；观察六味地黄丸对苯肾上腺素（PE）刺激的胸主动脉血管环收缩反应的影响和乙酰胆碱（Ach）、硝普钠（SNP）刺激的胸主动脉血管环舒张反应的影响；并检测血管组织中HO-1 mRNA及其蛋白的表达。结果：与正常组比较，模型组大鼠SBP升高，主动脉血管环对Ach刺激的舒张反应减弱、对PE刺激的收缩反应增强（P＜0.05）；血管组织中HO-1 mRNA及其蛋白表达水平差异无统计学意义（P＞0.05）。与模型组比较，六味地黄丸组大鼠主动脉血管环对Ach刺激的舒张反应增强，对PE刺激的收缩反应减弱，且血管组织中HO-1 mRNA及其蛋白表达水平升高（P＜0.05或P＜0.01）。与六味地黄丸组比较，联用药组大鼠SBP持续升高、对Ach刺激的舒张反应持续减弱（P＜0.05），血管组织中HO-1 mRNA及其蛋白水平差异无统计学意义（P＞0.05）。各组大鼠间比较，心率差异均无统计学意义（P＞0.05）。结论：六味地黄丸可改善糖尿病模型大鼠血管反应性失调，这可能与其高表达血管组织中HO-1有关。

Abstract

OBJECTIVE： To investigate the effects of Liuwei dihuang pills on heme oxygenase 1 （HO-1） in vascular tissue of diabetic model rats. METHODS： Rats were randomly divided into normal group， model group， Liuwei dihuang pills group （14 g/kg， ig， s.i.d） and HO-1 inhibitor zinc protoporphyrin （ZPP， 10 μmol/kg， ip， q.o.d）+Liuwei dihuang pills （14 g/kg， ig， s.i.d） group， with 12 rats in each group. Except for normal group， those groups were given streptozotocin intraperitoneally to induce diabetic model. After modeling， treatment groups were given relevant medicines， and normal group and model group were given normal saline intraperitoneally every 2 days for consecutive 4 weeks. The systolic blood pressure （SBP） of caudal artery and heart rate in rats were detected； the effects of Liuwei dihuang pills on the contraction response of thoracic aortic rings （TARs） irratated by phenylephrine （PE） were observed as well as the effects on the relaxation response of TARs irratated by acetylcholine （Ach） and sodium nitroprussiate （SNP）； mRNA and protein expression of HO-1 in vascular tissue were examined. RESULTS： Compared with normal group， SBP of model group increased， and the relaxation response of TARs to Ach irritation decreased while the contraction response of TARs to PE irritation increased （P＜0.05）. There was no statistical significance in mRNA and protein expression of HO-1 in vascular tissue （P＞0.05）. Compared with model group， the relaxation response of TARs to Ach irritation increased in Liuwei dihuang pills group， while the contruction response of TARs to PE irritation decreased； mRNA and protein expression of HO-1 increased in vascular tissue （P＜0.05 or P＜0.01）. Compared with Liuwei dihuang pills group， SBP of drug combination group increased continuously， while the relaxation response of TARs to Ach irritation decreased continously （P＜0.05）. There was no statistical significance in mRNA and protein expression of HO-1 in vascular tissue （P＞0.05）. There was no statistical significance in heart rate among those groups （P＞0.05）. CONCLUSIONS： Liuwei dihuang pills can improve vascular reactivity disorder of diabetic model rats， which is likely related to the expression up-regulation of HO-1 in vascular tissue.

关键词

六味地黄丸糖尿病血红素加氧酶1血管功能大鼠

Keywords

Liuwei dihuang pillsDiabetesHeme oxygenase 1Vascular functionRats

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Chongqing Key Laboratory of Translational Medicine in Major Metabolic Diseases,The First Affiliated Hospital of Chongqing Medical University

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