OBJECTIVE： To establish a method for the simultaneous determination of （R，S）-goitrin， chlorogenic acid， luteoloside and isochlorogenic acid A in Xiaoer ganmao granule. METHODS： HPLC was performed on the column of Hedera C18 with mobile phase of acetonitrile-0.1％ formic acid aqueous at a flow rate of 1.0 ml/min， the detection wavelength was 254 nm， 330 nm and 370 nm， column temperature was 40 ℃， and the injection volume was 5 μl， RESULTS： The linear range was 6.6-105 μg/ml for （R，S）-goitrin （r＝0.999 9）， 9-140 μg/ml for chlorogenic acid （r＝0.999 9），9-144 μg/ml for luteoloside （r＝0.999 8） and 9-138 μg/ml for isochlorogenic acid A （r＝0.999 6）； the limits of quantitation were 330 ng， 450 ng， 450 ng and 450 ng， limits of detection were 66 ng， 90 ng， 90 ng and 90 ng， respectively； RSDs of precision， stability and reproducibility tests were lower than 3％； recoveries were 95.01％-98.77％（RSD＝1.48％，n＝6），95.14％-98.91％（RSD＝1.52％，n＝6）， 95.11％-97.54％（RSD＝0.93％，n＝6） and 95.58％-99.63％（RSD＝1.73％，n＝6）. CONCLUSIONS： The method is simple and accurate， and suitable for the simultaneous determination of （R，S）-goitrin， chlorogenic acid， luteoloside and isochlorogenic acid A in Xiaoer ganmao granule.