OBJECTIVE： To establish a method for the simultaneous determination of psoralen， isopsoralen， epimedin B， epimedin C and icariin in Chanlong dingchuan mixture. METHODS： HPLC was performed on the column of Welch Materials C18 with mobile phase of acetonitrile - methanol （1 ∶ 1，V/V）-water （gradient elution） at a flow rate of 0.9 ml/min， the detection wavelength was 246 nm （psoralen， isopsoralen） and 270 nm （epimedin B， epimedin C and icariin）， column temperature was 30 ℃， injection volume was 20 μl. RESULTS： The linear range was 8.24-164.80 μg/ml for psoralen （r＝0.999 7）， 5.15-103.00 μg/ml for isopsoralen （r＝0.999 3），4.06-81.20 μg/ml for epimedin B （r＝0.999 6）， 5.88-117.60 μg/ml for epimedin C（r＝0.999 5） and 4.90-98.00 μg/ml for icariin （r＝0.999 8）； the limits of quantitation were 0.385 μg/ml， 0.179 μg/ml ， 0.124 μg/ml， 0.218 μg/ml and 0.348 μg/ml， limits of detection were 0.127 μg/ml， 0.059 μg/ml ，0.041 μg/ml， 0.072 μg/ml and 0.115 μg/ml； RSDs of precision， stability and reproducibility tests were lower than 2.0％； recoveries were 97.93％-100.06％ （RSD＝0.80％，n＝6）， 96.91％-100.16％ （RSD＝1.37％，n＝6）， 96.95％-99.63％ （RSD＝0.98％，n＝6）， 96.69％-99.33％ （RSD＝1.03％，n＝6） and 96.76％-98.53％ （RSD＝0.70％，n＝6）， respectively. CONCLUSIONS： The method is simple and reliable， and suitable for the simultaneous determination of psoralen， isopsoralen， epimedin B， epimedin C and icariin in Chanlong dingchuan mixture.