OBJECTIVE： To establish a method for the simultaneous determination of vitamin B6， hydrochlorothiazide， rutin， promethazine hydrochloride and reserpine in Compound trizin and tutinum tablet. METHODS： HPLC was performed on the column of Agilent Zorbax SB-C18 with mobile phase of acetonitrile-0.2％ phosphoric acid （gradient elution） at a flow rate of 1.5 ml/min， detection wavelength was 291 nm （0-3 min， vitamin B6），273 nm（3-5 min， hydrochlorothiazide），251 nm（5-11 min， rutin， promethazine hydrochloride） and 218 nm（11 min， reserpine）， column temperature was 30 ℃， and injection volume was 20 μl. RESULTS： The linear range was 84.0-1 344.0 μg/ml for vitamin B6（r＝0.999 8），171.0-2 736.0 μg/ml for hydrochlorothiazide（r＝0.999 2），428.0-6 848.0 μg/ml for rutin（r＝0.999 9），173.5-2 776.0 μg/ml for promethazine hydrochloride（r＝0.999 5） and 5.03-80.48 μg/ml for reserpine（r＝0.999 1）； limits of quantification were 18.80，14.92， 25.70， 12.20， 4.99 ng， limits of detection were 5.54， 4.50， 8.04， 3.66 1.50 ng， respectively； RSDs of precision， stability and reproducibility tests were lower than 1.0％； recoveries were 98.20％-100.60％（RSD＝0.89％，n＝6），99.11％-100.28％（RSD＝0.39％，n＝6）， 99.07％-100.12％（RSD＝0.38％，n＝6）， 98.27％-99.70％（RSD＝0.50％，n＝6）， 97.41％-99.40％（RSD＝0.88％，n＝6）， respectively. CONCLUSIONS： The method is simple， accurate with high sensitivity and good reproducibility， and can be used for the simultaneous determination of vitamin B6， hydrochlorothiazide， rutin， promethazine hydrochloride and reserpine in Compound trizin and tutinum tablet.