OBJECTIVE： To establish a method for the simultaneous determination of scutellarin and linarin in Scoparia dulcis. METHODS： HPLC was performed on the column of Phenomenex C18 with mobile phase of acetonitrile-0.05％ phosphoric acid （gradient elution） at a flow rate of 1.0 ml/min， the detection wavelength was 330 nm， column temperature was 30 ℃， and the injection volume was 10 μl. RESULTS：The linear range was 0.015 8-0.316 8 mg/ml for scutellarin （r＝0.999 9） and 0.000 5-0.010 4 mg/ml for linarin （r＝0.999 7）； RSDs of precision， stability and reproducibility tests were lower than 3％； recoveries were 99.56％-102.38％（RSD＝1.07％，n＝6） and 95.14％-98.29％（RSD＝1.24％，n＝6）， respectively. CONCLUSIONS： The method is simple with good stability and reprodicibility，and can be used for the simultaneous determination of scutellarin and linarin in  S. du- lcis.