OBJECTIVE： To establish a method for the simultaneous determination of paeoniflorin and paeonol in Dirda pill，and provide reference for improving its quality control standard. METHODS： HPLC was performed on the column of ZORBAX C18 with mobile phase of acetonitrile-0.1％ phosphoric acid （gradient elutio） at a flow rate of 0.8 ml/min， detection wavelength was 230 nm（paeoniflorin）， 274 nm（paeonol）， columne temperature was 35 ℃， injection volume was 20 μl. RESULTS： The linear range was 0.514 8-1.544 5 μg for paeoniflorin （r＝0.999 2） and 0.643 2-1.960 4 μg for paeonol（r＝0.999 8）； the limits of quantification were 0.135 6， 0.126 4 μg， limits of detection were 0.067 8， 0.063 2 μg； RSDs of precision， stability and reproducibility tests were lower than 2％； recoveries were 95.78％-97.27％（RSD＝0.62％，n＝6） and 97.38％-98.38％ （RSD＝0.42％，n＝6）. CONCLUSIONS： The method is simple with good reprosucibility and high sensibility， and can be used for the simultaneous determination of paeoniflorin and paeonol in Dirda pill.