OBJECTIVE： To study the effects of puerarin （Pue） on the apoptosis of H9c2 myocardial cell induced by epirubicin（Epi）. METHODS： The H9c2 myocardial cells were divided into blank control group， model group， positive control group （10     μmol/L dexrazoxane） and Pue low-dose， medium-dose and high-dose groups （10， 50， 100 μmol/L）， with 5 wells in each group. Except blank control group didn’t received any treatment， other groups were treated with Epi （1.0 μmol/L） and relevant medicine. 24 h later， survival rate of myocardial cell was measured by CCK-8 kit； apoptotic morphology was observed by Hoechst 33258 fluorescent staining； apoptotic rate of myocardial cell was determined by flow cytometry； the protein expression of Bax， Bcl-2 and Cleaved-caspase-3 were assayed by Western blot； SOD activity and MDA content were assayed by colorimetric kit. RESULTS： Compared with blank control group， apoptotic body was obviously found in cell nucleus of model group； survival rate， the expression of Bcl-2 and SOD activity were decreased； while the apoptotic rate， the expression of Bax and Cleaved-caspase-3， Bax/Bcl-2 ratio and MDA content were increased. Compared with model group， the morphology of cell nucleus was complete in positive control group and Pue medium-dose and high-dose groups； survival rate， the expression of Bcl-2 and SOD activity were increased； while apoptotic rate， the expression of Bax and Cleaved-caspase-3， Bax/Bcl-2 ratio and MDA content were decreased， with statistical significance （P＜0.05）. There was no statistical significance in other indexes （P＞0.05）. CONCLUSIONS： Pue could mitigate cardiac toxicity of Epi via inhibiting apoptotic signaling pathway and depressing oxidative damage.