OBJECTIVE： To establish a method for the simultaneous determination of coumarin compounds in Yifu pill. METHODS： HPLC was performed on the column of Inertsil ODS-3 with mobile phase of acetonitrile-water（49 ∶ 51，V/V） at a flow rate was 1.0 ml/min， the detection wavelength was 320 nm， column temperature was 30 ℃， injection volume was 20 μl. RESULTS： The linear range was 5.16-206.4 μg/ml for osthole （r＝0.999 9），37.40-1 495.89 μg/ml for isoimperatorin（r＝0.999 9） and 9.95- 318.34 μg/ml for columbianadin （r＝0.999 9）； RSDs of precision， stability and reproducibility tests were lower than 3.0％； recoveries were 96.05％-103.19％（RSD＝2.18％，n＝9） for osthole，96.90％-103.09％（RSD＝2.07％，n＝9） for isoimperatorin，95.50％-103.57％（RSD＝2.15％，n＝9） for columbianadin. CONCLUSIONS： The method is simple with good precision， stability and reproducibility， and can be used for the simultaneous determination of coumarin compounds in Yifu pill.