OBJECTIVE: To establish the quality standard for Gardenia jasminoides. METHODS: The moisture, total ash and extract were determined. HPLC was used for contents determination of geniposide, rutin, crocinⅠand crocinⅡ: the column was Waters Xbridge-C18 with mobile phase of acetonitrile-0.2% phosphoric acid (gradient elution, gardenia and rutin), methanol-water (45 ∶ 55,V/V, crocinⅠ, crocinⅡ) at a flow rate of 1.0 ml/min; detection wavelength was 256 nm for gardenia and rutin, 440 nm for crocinⅠ, crocinⅡ; column temperature was 30 ℃; injection volume was 10 μl for gardenia and rutin, 5 μl for crocinⅠ, crocinⅡ. RESULTS: The moisture total ash and ethanol-soluble extract of G. jasminoides were 5.8%-8.4%,3.7%-5.9% and 29.5%-37.9%, respectively. The linear range was 162.08-1 620.84 μg/ml for geniposide (r=0.999 9), 2.07-20.72 μg/ml for rutin (r=0.999 9), 8.04-80.41 μg/ml for crocinⅠ(r=0.999 9) and 1.05-10.53 μg/ml for crocinⅡ(r=0.999 9); RSDs of precision, stability and reproducibility test were lower than 2%; recoveries were 99.33%-101.43% (RSD=1.09%,n=6), 97.97%-101.83% (RSD=1.39%,n=6), 97.97%-101.30%(RSD=1.36%,n=6) and 98.65%-103.04% (RSD=1.84%,n=6). CONCLUSIONS: The established standard can be used for the quality control of G. jasminoides commercially available.