OBJECTIVE： To analyze the distribution characteristics and drug resistance of pathogens in Shandong Dong’e and to provide reference for local physicians to choose antimicrobial agents. METHODS： Detected pathogens in Dong’e county  of Shandong province during Jan. 2014-Dec. 2015 were analyzed statistically in respects of type， distribution and drug resistance. RESULTS： A total of 25 562 specimens were collected from Dong’e county of Shandong province during 2014-2015， and there were 6 302 positive samples with positive rate of 24.65％. The samples with high positive rate included respiratory samples （positive rate of 34.52％） and secretion， pus （positive rate of 33.79％）. A total of 6 350 pathogens were detected， among which there were 3 707 strains of Gram-positive bacterias （58.38％）， mainly including Escherichia coli， Pseudomonas aeruginosa and Klebsiella pneumonia； 2 042 strains of Gram-negative bacterias （32.16％）， mainly including Staphylococcus aureus and Streptococcus pneumonia； 601 strains of fungus. E. coli， S. aureus， P. aeruginosa， K. pneumonia， Acinetobacter baumannii and Proteus mirabilis mainly distributed in ICU， and S. pneumonia and Hemophilus influenza mainly distributed in pediatric department. Sensitive rates of E. coli and K. pneumonia to piperacillin sodium and tazobactam sodium， imipenem， amikacin and tigecycline were higher than 90％； the strain resistant to or intermediate to tigecycline was not found. Sensitive rate of P. aeruginosa to aminoside antimicrobial agents was higher than 90％. Resistant rates of A. baumannii to various antimicrobial agents were higher than 70％， and resistant rate of it to minocycline was 96.67％. S. aureus was sensitive to vancomycin， quinupristin/dalfopristin and tigecycline， and S. pneumonia was sensitive to rifarnpin， linezolid and vancomycin， with sensitive rate of 100％. A total of 264 strains of MRSA were detected， and detection rate was 39.28％； a total of 200 strains of penicillin-resistant S. pneumonia were detected， and detection rate was 35.13％. CONCLUSIONS： The samples from Dong’e county  of Shandong province during 2014-2015 were mainly respiratory samples， detected pathogens were mainly Gram-negative bacterias and resistant rate of them were lower than related domestic reports. The distribution characteristics and drug resistance of pathogens should be considered before using antimicrobial agents to improve the rationality and pertinence of drug use.