OBJECTIVE： To develop a new way for the extration and determination of naringin in Davallia mariesii. METHODS： Naringin in D. mariesii was extracted by ASE350 accelerated solvent extraction system； HPLC was performed for the content determination of naringin in D. mariesii， the column was Kinetex XB-C18 with mobile phase of methanol-5％ acetic acid（25 ∶ 75，V/V） at a flow rate of 0.8 ml/min， detection wavelength was 283 nm， column temperature was 40 ℃， and injection volume was 10 μl. RESULTS： The linear range of naringin was 0.073 0-0.730 0 μg（r＝0.999 9）； the limit of quantitation was 0.73 μg， the limit of detection was 0.022 μg； RSDs of precision， stability and reproducibility tests were lower than 2％； recovery was 98.92％-100.85％ （RSD＝0.72％，n＝6）. CONCLUSIONS： The method is simple， accurate and specific， and can be used for the content determination of naringin in D. Mariesii.