OBJECTIVE：To establish a method for the contents determination of astragalosidesⅠ，Ⅱ，Ⅲ，Ⅳ，lobetyolin and formononetin in Shenqi fuzheng injection. METHODS： HPLC was performed. The detector was evaporative light scattering detector， column was Ultimate XB-C18 with mobile phase of acetonitrile-0.5％ formic acid （gradient elution） at a flow rate of 1.2 ml/min， column temperature was 30 ℃， and injection volume was 10 μl. RESULTS： The linear range was 0.62-5.67 μg for astragalosidesⅠ（r＝0.999 6）， 0.78-6.31 μg for astragalosidesⅡ（r＝0.999 6）， 0.36-3.48 μg for astragalosidesⅢ（r＝0.999 7）， 0.81-6.72 μg for astragalosides Ⅳ（r＝0.999 5）， 0.82-7.03 μg for lobetyolin （r＝0.999 8） and 0.58-6.62 μg （r＝0.999 7） formononetin； limit of quantitation was 1.21，0.15，0.12，0.03，0.12，0.17 μg/ml； limit of detection was 0.35， 0.35， 0.04， 0.01， 0.03， 0.04 μg/ml； RSDs of precision， stability and reproducibility tests were lower than 2％；recoveries were 95.1％-101.1％（RSD＝2.0％，n＝9）， 95.2％-100.7％（RSD＝1.9％，n＝9）， 95.8％-100.2％ （RSD＝1.4％，n＝9），96.2％-100.6％（RSD＝1.7％，n＝9），96.6％-101.2％（RSD＝1.4％， n＝9） and 95.9％-99.5％（RSD＝1.2％，n＝9）， respectively. CONCLUSIONS： The method is simple and accurate， and can be used for the simultaneous determination of astragalosidesⅠ，Ⅱ，Ⅲ，Ⅳ， lobetyolin and formononetin in Shenqi fuzheng injection.