OBJECTIVE： To establish a method for the simultaneous determination of narirutin， naringin， hesperiden and neohesperiden in Weisu granule. METHODS： UPLC was performed on the column of ACQUITY UPLC BEH C18 with mobile phase of acetonitrile-0.2％ Phosphoric acid aqueous（gradient elution） at a flow rate of 0.40 ml/min， the detection wavelength was 284 nm， the column temperature was 30 ℃， and the injection volume was 1 μl. RESULTS： The linear range was 9.38-93.75 μg/ml for narirutin（r＝0.999 7）， 32.25-322.50 μg/ml for naringin（r＝0.999 7）， 11.25-112.50 μg/ml for hesperiden（r＝0.999 9） and 11.88-118.75 μg/ml for neohesperidin（r＝0.999 8）；limits of quantitation were 20 ng， 18 ng，18 ng and 18 ng，the limits of detection were 6 ng，5 ng ，5 ng and 5 ng， respectively； RSDs of precision， stability and reproducibility tests were lower than 2.0％； recoveries were 96.24％-103.12％ （RSD＝2.45％，n＝6）， 98.43％-102.10％ （RSD＝1.42％，n＝6）， 96.10％-101.41％ （RSD＝2.07％，n＝6） and 95.57％-99.06％ （RSD＝1.44％，n＝6）， respectively. CONCLUSIONS： The method is rapid and efficient， and suitable for the simultaneous determination of narirutin， naringin， hesperiden and neohesperiden in Weisu granule.