OBJECTIVE： To establish a method for the simultaneous determination of andrographolide， quercetin-3-O-glucoside， dehydroandrographolide and kaempferol-3-O-glucoside in Compound chuanxinlian tablet. METHODS： HPLC was performed on the column of Phenomenex C18 with mobile phase of acetonitrile - 0.1％ phosphoric acid aqueous（gradient elution） at a flow rate of 1.0 ml/min， the detection wavelength was 225 nm for andrographolide， 335 nm for quercetin-3-O-glucoside and 254 nm for dehydroandrographolide and kaempferol-3-O-glucoside， column temperature was 25 ℃， and the volume injection was 10 μl. RESULTS： The linear range was 0.111-2.770 μg for andrographolide（r＝0.999 9），0.064-1.605 μg for quercetin-3-O-glucoside （r＝0.999 9）， 0.086-2.138 μg for dehydroandrographolide（r＝0.999 9） and 0.050-1.260 μg for kaempferol-3-O-glucoside（r＝0.999 9）； the limits of quantitation were 0.48 ng， 0.88 ng，0.93 ng and 1.03 ng， the limits of detection were 0.79 ng， 1.15 ng，0.96 ng and 1.07 ng，respectively；RSDs of precision， stability and reproducibility tests were lower than 2.0％；recoveries were 96.57％-100.30％（RSD＝1.3％，n＝9），95.04％-98.87％ （RSD＝1.3％，n＝9）， 95.74％-102.92％ （RSD＝2.4％，n＝9） and 96.73％-100.95％（RSD＝1.6％，n＝9），respectively. CONCLUSIONS： The method is sensitive， reliable and reproducible， and can be used for the simultaneous determination of andrographolide， quercetin-3-O-glucoside， dehydroandrographolide and kaempferol-3-O-glucoside in Compound chuanxinlian tablet.