OBJECTIVE： To discuss the drug resistance situation and influential factors of sputum culture positive tuberculosis patients in a specialized subject hospital. METHODS： The information of sputum culture positive tuberculosis inpatients were collected from Heze infectious disease hospital during Jan. 1st 2014 to Apr. 30th 2015； the results of drug sensitive test were analyzed retrospectively by using absolute concentration method. RESULTS： Among 259 patients， 137 cases were drug-resistant（52.90％）， among including 69（26.64％） mono-drug resistant cases， 52（20.08％） poly-drug resistant cases， 15（5.79％）multi-drug resistant cases and 1（0.39％）extensively drug resistant case； streptomycin （31.66％） and amikacin （15.44％） were drugs with the highest drug-resistant rate； there was no statistical significance in resistant rate between different genders and ages（P＞0.05）， the rate of drug resistance in first-treatment patients was 47.21％， and that of the re-treatment patients was 70.97％， the difference was statistical significant（P＜0.05）. In addition to ethambutol and kanamycin， other all had mono-drug resistance， there were 27 combinations of poly-drug resistance and 9 combinations of multi-drug resistance. Logistic regression analysis showed that treatment history was a risk factor for drug-resistance， the risk of re-treatment patient was 2.734 times of first-treatment patients. CONCLUSIONS： Sputum culture positive tuberculosis inpatients patients have high drug resistance and complex spectrum in the hospital； we should pay great attention to the risk of drug resistance in senile patients and re-treatment patients， and rational use of streptomycin， amikacin， isoniazid and capreomycin.